Three different types of modelling requests (automated mode, alignment mode, project mode) are provided, which differ in the amount of user intervention. This fully automated protein structure server is accessible via the ExPasy web server to match models online with multiple templates and established database. Protein homology models can be computed by the SWISSMODEL server homology modelling pipleline. Zebrafish tbx22 mRNA expression mirrors mammalian TBX22 expression and is consistent with early patterning of the vertebrate face. The two zebrafish tbx22 splice isoforms, tbx22a and tbx22b, encode proteins of 444 and 400 amino acids, respectively. The DiGeorge pattern of defects has been observed in the van gogh/tbx1 zebrafish mutant. While TBX1 has been shown to regulate palatogenesis, a similar complex defect is found in DiGeorge syndrome. TBX22 is a part of the TBX1 subfamily which also consists the genes TBX1, 10, 15 18 and 20. The human form of TBX22 has 5 transcripts with 2 involved in protein coding. Characterization of several Tbx genes in humans suggests a role for these genes in craniofacial development. Mutations in human TBX22 cause X-linked cleft palate with anklyoglossia syndrome affecting palatogenesis. All the T-box genes share a conserved DNA domain which is homologous, encoding the T-box. The T-box genes are an ancient family of transcription factors that are well conserved throughout all metazoans Genes associated with T-box are significantly involved in the regulation of the developmental processes of early mesoderm induction, specification, patterning and somite formation in many organisms. This gene is a member of a phylogenetically conserved family of genes, which share a common DNA-binding domain T-box. T-box, tbx22, Transcription Factors, Genomatix, Protein Prediction, Superimposed Models These studies also support zebrafish as a predictive model for numerous developmental pattering events in higher vertebrates. Bioinformatic-Proteomics and protein structural analysis approaches shown here explain in detail the relationship between the Human and Zebrafish tbx22 Gene-Protein-Transcrip- tion factor. Transcription factor and promoter element analysis with Transcriptome Viewer, Gene 2 Promoter and Genomeinspector reveal a high degree of homology between the 2 organisms. Additional supporting data from Genomatix MATBASE, MATINSPECTOR show higher matrix family scores for BRAC (Brachury gene mesoderm developmental factor) in Human and Zebrafish. Superimposing the predicted protein structures reveals conserved domains between the human and zebrafish proteins. Protein homology prediction shows more than 65% between the 2 organisms. Secondary and tertiary structures of the proteins are predicted using standard structure prediction software’s like Phyre 2, Predict Protein, SWISSMODEL, PSIPRED and the homology of the proteins were compared to each other. This study examines the similarity in the developmental pattern influenced by the transcription factor TBX22 and tbx22 in H. The gene tbx 22 is a member of a phylogenetically conserved family of genes, which share a common DNA binding domain: T box. Received 16 February 2016 accepted 23 March 2016 published 30 March 2016īiological functions of proteins play a key role in the development of any organism. Department of Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA, USA
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